polyclonal goat anti cd14 antibody  (Novus Biologicals)

Journal: bioRxiv

Article Title: Low grade inflammation in the epileptic hippocampus contrasts with explosive inflammation occurring in the acute phase following status epilepticus in rats: translation to patients with epilepsy

doi: 10.1101/2021.03.25.436701

Figure Lengend Snippet: ( a , b ) ITGAM was detected in the dentate gyrus of rats subjected to pilocarpine-induced SE at P42 and sacrificed 24 hours later. a Orange arrows depict “round-shaped cells” within the brain parenchyma, intermingled to activated resident microglial cells. b Firm adhesion of round-shaped cells to endothelial cells is illustrated. c Double fluorescent immunolabeling of ITGAM (Green) and CD14 (Red) in the hippocampus shows that almost all ITGAM+ cell infiltrates are monocytes/macrophages (CD14+). Scale bars: a,b: 50 µm; c: 20 µm

Article Snippet: For immunohistofluorescent detections, free-floating sections (40 μm thick) from paraformaldehyde-fixed tissue were incubated with a rabbit polyclonal anti-GFAP antibody (1:1,000; AB5804, Chemicon) to label astrocytes, a mouse monoclonal anti-ITGAM antibody (1:1,000; CBL1512Z, Chemicon) to detect microglia and immunocompetent cells, a goat polyclonal anti-CD14 antibody (1:1,000; sc-5749, Santa-Cruz) to detect monocytes, a mouse monoclonal anti-NeuN antibody (1:1,000; MAB-377, Chemicon) to label neurons, and finally rabbit polyclonal anti-IL1β antibody (1:200; #250716, Abbiotec).

Techniques: Immunolabeling